Endothelial Tube Formation Assay (In Vitro Angiogenesis)

Angiogenesis assay human - HUVEC

Experiment
Angiogenesis assay human - HUVEC
Product
Endothelial Tube Formation Assay (In Vitro Angiogenesis) from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
- 200 µL of thawed ECM gel solution to each well.

- 600 uL of cell suspension was added onto the solidified ECM gel and incubated the assay plate at 37ºC for18 hrs.

Publication protocol

Angiogenesis in vitro was performed utilizing the endothelial tube formation assay kit (Cell Biolabs, CA), according to the instruction. The extracellular matrix gel was prepared from Engelbreth-Holm-Swarm tumor cells (200 μl/ well). HUVECs (ten thousand cells/well) infected with retroviruses or cultured with CM from PANC-1 cells that were infected with the indicated retroviruses. The mixture was added onto solidified extracellular matrix gel in 600 μl medium. After incubation for 18 h, endothelial cell tube formation was assessed and tube number was counted.


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Discussion

Discussion

5 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

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Manufacturer protocol

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